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We also provide evidence that the use of R genes from wild relatives can increase disease resistance in potato and that R genes from wild relatives can transfer effectively in potato. These results suggest that the resistance from wild relatives can provide an additional and important source of resistance to late blight in potato, and that wild relatives represent a potential source of useful R genes for improving the response of potato to late blight.
We have also characterized the three R gene stacking event's genetic structure by sub-molecular characterization of the three R genes and detection of the R-gene stacking event in the progeny of the 'Desiree' × 'Victoria' transgenic line. A genetic map was developed and the R gene loci were mapped to the tetraploid potato genome assembly of 'Desiree' and 'Victoria'. A single event was detected, which contained stacks of Rpi-vnt1.1 and Rpi-blb2 for which we called 'papains'. The 'papains' event was confirmed by the presence of gene specific sequences and the absence of the poly-A tail. In addition, the alleles at the Rpi-vnt1.1 locus were verified to be different from those in the original donor material. Thus, the 'papains' event is an independently inherited derivative of the donor event and may be useful in enhancing the disease resistance and productivity of the wild relatives.
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